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ePaper created 2017-01-04, 09:21:41 | version 1.38.0
| research 12 laser 4 2016 – – Dyeing of the sections with 1 % Uranyl acetate in methanol and 1 drop of acetic acid for 10' – – Microscope: Zeiss EM 906. Results Light microscopy and vitality test The cells received thermal treatment at tempera- tures ranging from 37 °C to 60 °C and varying inter- mediate temperature levels. Light microscopy exam- inations showed significant morphological changes at temperatures from 46.5 °C ± 0.5 °C. Attemperaturesfrom37 °Cto45 °C,allcellsexhib- ited a green calcein fluorescence. At temperatures of 46 °C and above, lethal results were detected in some of the cells that had undergone thermal treatment. The number of lethal cells increased in correspon- dence to a rise in temperature. At temperatures of 46 °C to 56.5 °C, the number of lethal cells had almost doubled 24 h after thermal treatmentincomparisontothenumberoflethalcells one hour after thermal treatment (Table 1, Fig. 1). Starting at 56.5 °C, this phenomenon ceased, with Fig. 3 Fig. 4 Fig. 5 Fig. 3: Control cells exhibited a normal appearance at 37 °C under REM. Cell processes, microvilli-like structures on the cell surface (their numbers seems to depend on the level of cell activity) as well as the elongated cell shape are clearly visible. Fig. 4: REM: Thermally treated DPSC showed external signs of cellular damage at 46.5 °C: The cell usually changes its elongated shape and starts to round. At 50 °C, an increased rounding can be observed. The cell seems to contract so fast that a part of the cytoplasm processes tears off (arrows). The surface structure of the cells is effected as appearance and number of microvilli change. Fig. 5: REM: Thermal treatment at 60 °C. While the exterior shape remains mostly intact, their surface does not exhibit any structuring anymore. 42016