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ePaper created 2017-01-04, 09:21:41 | version 1.38.0
| research 10 laser 4 2016 Thermal damage behaviour of human dental pulp stem cells Authors: Prof. Dr Karsten König & Dr Anton Kasenbacher, Germany Objective This study was designed to examine the influence of temperatures ranging from 37 to 65 °C on the cell morphology of DPSC stem cells via light and electron microscopy,asynthesisofHeatShockProteins(HSP) with fluorescence-marked antibodies and vitality via the Life/Dead Fluorescence Kit. Material and methods DPCS were cultivated at 37 °C and 5 % CO2 in sterile cell chambers (MiniCeM, JenLab GmbH, Jena, Germany). The cells were irrigated with pre-heated culture medium (Eagle’s MEM, Gibco BRL, Paisley, Scotland, 37 °C) with 20 % FCS, 2 mM L-Glutamin and100 µML-Ascorbate-2-Phosphateinordertore- move cellular debris previously to the temperature trials. Filling the chamber with the culture medium followed and a preheated water bath of different temperatures was introduced. Up to an incubation temperature of 46 °C, the experiments were con- ducted with temperatures rising every 2 °C and 0.5 °C in the sensitive temperature scale of 46 °C to 58 °C.Inaddition,trialserieswerecarriedoutat60 °C Temperature Letality % °C 1 h 24 h 37 0 0 39 0 0 42 0 0 45 0 0 46 0,5 2 47 10 17 48.5 18 29 50 17 27 55 24 59 56.5 48 54 58 100 100 60 100 100 65 100 100 Table 1 Fig. 1 Table 1: Life/Dead Assay 1 hour und 24 hours after thermal treatment. Fig. 1: Vitality test of thermally treated DPSC. 42016 3700 3900 4200 4500 460,52 471017 48.51829 501727 552459 56.54854 58100100 60100100 65100100 Table 1 Fig. 1