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Fig. 4a Fig. 4b Fig. 4c Fig. 5 Fig. 6 Figs. 4a–c: Remove clot from tube and separate clot from red blood cells. Fig. 5: Specially designed kit (Xpression™ box) to compress L-PRF clots into L-PRF membranes with a consistent thickness of 1 mm. A piston and cylinder assembly (right) can be used for the creation of L-PRF plugs, suitable for filling extraction sockets. Fig. 6: L-PRF membranes after gentle compression: the red area of the membrane represents the face side where most leucocytes, platelets and stem cells are concentrated. opposite to each other to ensure the centrifuge is properly balanced. Close the cover and set the timer to one minute. Press START and allow the centri- fuge to run for one minute, it will then come to a full stop and the cover will pop open. While it is spinning for one minute collect the third and fourth tube of blood from patient, and repeat the procedure for the other tubes. – Centrifugation should be at 408 g (2,700 rpm using the IntraSpin™ centrifuge, for at least 12 minutes (start timing after loading the centrifuge with the last two tubes, Fig. 2). – After 12 minutes of centrifugation (for patient taking anti- coagulant medication up to 18 minutes are rec- ommended) L-PRF clots are ready (Fig. 3). – Take the fibrin clots out of the tubes and separate them Protocol for preparation of L-PRF plugs – Place fibrin clots in the small white cylinder of the Xpression™ box. – Use the piston to carefully compress the clot, until holder is level to cylinder. – The viability for expressed plugs is 2.5 to 3 hours, as long as they are re-hydrated with exudate. Editorial note: To be continued in implants 2/18 with ap- plication approaches for open flap debridement and ridge preservation. For further information visit: www.ENHD2018.be from the red blood cells (Figs. 4a–c). contact Protocol for preparation of L-PRF membranes – Place fibrin clots in Xpression™ box for gentle com- pression by gravity (e.g. with light metal plate, Fig. 5). – Five minutes later the L-PRF membranes are ready for use (Fig. 6). – The viability for expressed membranes is 2.5 to 3 hours, as long as they are re-hydrated with exudate. Prof. Dr Marc Quirynen University Hospitals Leuven Periodontology & Oral Microbiology Kapucijnenvoer 7 blok a – box 7001 3000 Leuven, Belgium Tel.: +32 163 32483 marc.quirynen@kuleuven.be Author details 08 1 2018