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B a c t e r i a l c o l o n i z a t i o n o n d i f f e r e n t a b u t m e n t m a t e r i a l s Influence of abutment material and detersion protocol on bacterial adhesion: An in vitro study Luca Sbricoli,a Gianluca Paniz,a Davide Abate,b Alda Saldan,b Giorgio Palùb & Eriberto Bressana a Department of Neurosciences, School of Dentistry, University of Padua, Padua, Italy b Department of Molecular Medicine, University of Padua, Padua, Italy C o r r e s p o n d i n g a u t h o r : Dr. Luca Sbricoli Department of Neurosciences School of Dentistry University of Padua Via Giustiniani, 2 35100 Padua Italy T +39 04 98212040 sbricoli.luca@gmail.com H o w t o c i t e t h i s a r t i c l e : Sbricoli L, Paniz G, Abate D, Saldan A, Palù G, Bressan E. Influence of abutment material and detersion protocol on bacterial adhesion: An in vitro study. J Oral Science Rehabilitation. 2018 Mar;4(1):32–36. Abstract O b j e c t i v e In recent years, periimplantitis has been extensively studied as bone loss has been observed around dental implants. As a result of multiple factors, different materials might enhance different patterns of bacterial plaque accumulation. The purpose of this research was to assess bacterial adhe- sion to different abutments and define the efficacy of different detersion protocols in reducing bacterial adhesion. M e t h o d s Four kinds of prefabricated abutments were analyzed: machined pure titanium abutments without anodization, machined gold hue and pink hue anodized pure titanium abutments and zirconia abutments with tita- nium connectors. All of the (sterile) abutments were immersed in sepa- rate bacterial suspensions (Staphylococcus haemolyticus, Streptococcus pyogenes and Escherichia coli) and contaminated with 3 × 108 colony- forming units per mL of each bacterial species suspension. Then, the following detersion protocols were compared: no treatment represent- ing the internal control, 10 min rinsing with water, 10 min incubation in 0.05% chlorhexidine. The microbial abatement was determined by swab collection of abutment-attached microbes and swab streaking on specific culture plates in a semiquantitative manner. Microbial growth was deter- mined at 24 and 48 hours after inoculation. R e s u l t s Contaminated abutments that had not undergone any cleaning treatment displayed a microbial growth up to the third quadrant of the culture plate. Chlorhexidine rinsing completely removed bacterial contamination. No statistically significant differences were found in terms of bacterial adhe- sion and bacterial growth among the different types of abutments. C o n c l u s i o n All of the analyzed abutments displayed similar characteristics with regard to bacterial adhesion. A low concentration of chlorhexidine had a significant disinfectant activity, regardless of the type of abutment. K e y w o r d s Dental implant, implant abutment, anodization, bacterial contamination. 32 Volume 4 | Issue 1/2018 Journal of Oral Science & Rehabilitation