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M o u t h w a s h e s a n d b a c t e r i a o n s u t u r e t h r e a d s Fig. 1 Flowchart illustrating the study protocol. Fig. 1 C u l t u r e m e d i a a n d c o n d i t i o n s Bacteria were cultured in Petri dishes containing tryptone soy agar (TSA) as growth medium at an incubation temperature of 36 ± 1 °C. In this way, it was possible to obtain the growth of mesophilic bacteria. De Man, Rogosa and Sharpe (MRS) agar at 36 ± 1 °C allowed the growth of Lactobacillus spp. Bacteroides bile esculin (BBE) agar and brucella blood agar (BRU) with vitamin K and hemin, incubated at 36 ± 1 °C under anaer- obic conditions allowed the qualitative analysis of specific anaerobic strains. T e s t p r o c e d u r e a n d b a c t e r i a l s c o r i n g samples were thawed at room temperature. Each sample was cut into 3 segments of similar length, then each thread sample was subjected to analysis as follows. The control sample was represented by segments of a sterile, unused suture thread. Assessment of the absence/presence of bacte- rial load: Two segments of the thread were scraped on 2 culture plates containing agar- based broth media (TSA and MRS) and incubated for 48 h at 36 ± 1 °C in order to determine whether microbial load was present on the thread surface. The plates were visually inspected and bacterial load accordingly classi- fied as absent or present. Microbial load was assessed after suture removal using in vitro bacterial cultures. Suture thread samples were stored at -20 °C until microbiological analysis. Before testing, Enumeration of bacterial colonies: One segment was placed in a tube containing a diluent solution (buffered peptone water; BPW) for 1 h, then, fur- ther serial decimal dilutions were carried out. 26 Volume 3 | Issue 4/2017 Journal of Oral Science & Rehabilitation