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O z o n e t r e a t m e n t f o r d e n t i n a l h y p e r s e n s i t i v i t y thus facilitating the entry of minerals from the saliva and desensitizing agents containing fluo- ride.9, 10 S t u d y a i m The aim of the present study was to assess the effectiveness of ozone in reducing dentinal hyper sensitivity. The results obtained with ozone therapy were compared with those of an active control group, treated with a paint-on desensitizing agent. Materials and methods The prospective study was conducted at the Division of Oral Medicine and Pathology of the dental clinic of the Ospedale Maggiore (Trieste, Italy). All of the procedures performed were in accordance with the ethical standards of the institutional and national research committee and with the principles embodied in the Helsinki Declaration of 1975, as revised in 2013. The study included 40 patients between the ages of 21 and 85, selected according to the following criteria: 1. Inclusion criteria: – presence of dentinal hypersensitivity; – single or multiple dental elements; – dental elements affected by noncarious lesions of the enamel (abrasion, erosion, demineralization, white spot); – exposed root of a nonimplant prosthesis; – cooperative and able to define the pain experienced according to a numeric rating scale (NRS). 2. Exclusion criteria: – radiotherapy in the head neck region; – xerostomia; – carious lesions; and – Class V fillings. All of the enrolled patients were randomized into two groups, allocating one patient to the ozone group and the subsequent one to the paint group, until the predetermined number was reached. S t u d y d e s i g n sitivity. Then, through an objective examination, a dental hygienist recorded the presence of gin- gival recession and dental abrasion, performed periodontal screening and recording, and scored the patient according to the plaque index. All of the patients were asked to complete a question- naire regarding their food and oral hygiene habits and the severity of their dentinal hyper- sensitivity symptoms (Appendix A). The pain symptomatology was quantified, evoking the stimulus through the application of a jet of compressed air at the level of the affect- ed element. A numeric rating scale (NRS) was used, asking each patient to express the degree of pain on a scale of 0 to 10, in which 0 repre- sented the absence of pain and 10 the worst pain imaginable. Treatment with ozone The ozone group was treated with the medical ozone generator OZONE DTA (Sweden & Martina, Due Carrare, Italy), which produces ozone through the formation of an electromagnetic field. Treatment with ozone was planned for four sessions one week apart each (T1, T2, T3, T4), according to the following protocol: 1. evaluation and registration of the NRS (0–10) with a jet of compressed air; 2. cleansing of the affected element using a dental water brush mounted on a micromotor, without using an abrasive paste; 3. drying of the element with compressed air; 4. ignition and activation of the machinery, set to program No. 6; 5. application of the tip of the probe without contact with the dental element, but in a per- pendicular position with respect to the area of the tooth concerned, with continuous movement on the whole area for a total du- ration of 1 min; 6. evaluation and registration of the NRS (0–10) with a jet of compressed air. The patients were reassessed after 14 days (FU1) and monthly for six months (FU2, FU3, FU4, FU5, FU6, FU7) after the last ozone session. The evaluation was performed by recording the post-treatment hypersensitivity according to the NRS and by completion of the questionnaire. An example of a clinical case is provided in Figure 1. First, for all of the patients, a thorough clinical oral examination was performed in order to iden- tify the elements affected by dentinal hypersen- Treatment with paint For the paint group, the VivaSens desensitizing varnish (IvoclarVivadent, Schaan, Liechtenstein) Journal of Oral Science & Rehabilitation Volume 3 | Issue 2/2017 19