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ePaper created 2016-06-09, 15:16:05 | version 1.38.0
Journal of Oral Science & Rehabilitation 42 Volume 2 | Issue 2/2016 C o m p o s i t i o n o f P R P Composition of platelet-rich plasma gel: A Western blot analysis Pablo Galindo Moreno,a Gustavo Avila Ortiz,b Laura Torrecillas Martinez,a Juan Emilio Fernández Barbero,c Francisco O’Valled & Hom-Lay Wange a Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, Granada, Spain b Department of Periodontics, College of Dentistry, University of Iowa, Iowa City, Iowa, U.S. c Human Anatomy and Embryology Department, School of Medicine, University of Granada, Granada, Spain d Pathology Department, School of Medicine, and Institute of Biopathology and Regenerative Medicine, University of Granada, Granada, Spain e Department of Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, Mich., U.S. C o r r e s p o n d i n g a u t h o r : Dr. Pablo Galindo Moreno C/ Recogidas, 39 5º Izq 18005 Granada Spain T +34 958 52 0658 F +34 958 52 0658 pgalindo@ugr.es H o w t o c i t e t h i s a r t i c l e : Galindo Moreno P, Avila Ortiz G, Torrecillas Martinez L, Fernández Barbero JE, O’Valle F, Wang HL. Composition of platelet-rich plasma gel: a Western blot analysis. J Oral Science Rehabilitation. 2016 Jun;2(2):42–8. Abstract O b j e c t i v e Platelet-rich plasma (PRP) gel is an autogenous blood-derived material that may be used as a regenerative agent of oral structures. The regener- ative capacity of PRP is largely attributed to its composition, including manydifferentgrowthfactors.Thusfar,nostudyhasidentifiedthemole- cular content of this gel. Therefore, it was the purpose of this study to assessthepresenceofdifferentgrowthfactorsinPRPgel,usingtheWest- ern blot technique. M a t e r i a l s a n d m e t h o d s Bloodsampleswerecollectedfrom20healthydonorsandthenprocessed toobtainPRPgelsamples.TheWesternblottechniquewasusedtodeter- mine the presence of the following growth factors: vascular endothelial growthfactor(VEGF),platelet-derivedgrowthfactor(PDGF),transform- ing growth factor-beta (TGF-Ƶ1), fibroblast growth factor (FGF), insulin- like growth factor-1 (IGF-1) and epidermal growth factor (EGF). R e s u l t s Westernblotanalysisshowedpositiveelectrophoreticbandscorrespond- ing to molecular weights of the examined platelet growth factors. These bandswereobservedineverysampleofPRP,demonstratingtheirpresence as constituents of PRP gel. C o n c l u s i o n Data from our study showed that PRP gel contains varying amounts of VEGF, TGF-Ƶ1, bFGF, PDGF-A, IGF-1 and EGF. K e y w o r d s Platelet-rich plasma, growth factors, tissue engineering, wound healing, Western blotting. C/ Recogidas, 395º Izq T +34958520658 F +34958520658