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ePaper created 2016-03-24, 17:16:09 | version 1.38.0
implants 1 2016 | research 10 Figs. 3a–h: Phase I: Brand-new, sterile implants were used for the study. Implants supposed for SEM evaluation were initially kept in their original containers. The MRSA bacterial suspension was drawn in a sterile, disposable syringe (a) and applied directly on the respective implant in its original container (b & c). Subsequently, the shipment for immediate SEM analysis was carried out. Implants supposed for micro- biological testing were removed from their containers and placed directly into the MRSA bacterial suspen sion (d & e). After a one-minute inoculation period, the implants were removed and coated with Perisolv gel (f & g). After the exposure time specified by the manufacturer, the implants were introduced into the tube containing the nutrient medium and sent to the microbiological examination (h). Fig. 3a Fig. 3b Fig. 3c Fig. 3d Fig. 3e Fig. 3f Fig. 3g Fig. 3h Implant preparation for microbial investigations Immediately after application of the gel, the im- plants were introduced into tubes with a sterile nu- trient solution and sent to the Institute for microbi- ological analysis. The samples were processed in the Microbiological Institute by means of conventional (plate) cultivation. Scanning electron microscopic studies of the implants Some of the implants were investigated by scann ing electron microscopy (Institut Straumann AG). Results of Phase I—Decontamination procedure with contaminated whole implant bodies (Tab. 1) Scanning electron microscopic studies In some areas, where Perisolv had been applied, the “bacterial turf” on the implants was interrupted or rather dissolved/removed. Underlying areas, freed from bacterial turf, displayed an intact, unaltered implant structure. For implants only confronted with Perisolv without previous inoculation, no gel-in- duced change of the implant surface were observed. In summary, SEM analysis after treatment with the gel revealed no change of implant surface as and a partial dissolution of the inoculated bacterial layer. Microbiology Phase I investigations revealed bacterial inactiva- tion in the highest degree, remaining MRSA bacteria were detected in one test item of series B1 only. Summary of Phase I—Decontamination procedure with contaminated whole implant bodies The investigated gel is capable to induce a pro- nounced destruction of pathological bacteria pres- ent on implant surfaces without altering this implant surface structure. Phase II: Testing the effect of the antimicrobial gel on contaminated implants placed in a plastic jaw with a simulated peri-implant tissue defect After the first test phase to evaluate the principle suitability of the gel application, a second test phase was conducted. 12016