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ePaper created 2016-03-07, 22:38:59 | version 1.38.0
08 Volume 2 | Issue 1/2016 Journal of Oral Science & Rehabilitation S ta ph yloc oc c us a ureus and pe r i i mplant di se ase Identification of Staphylococcus aureus at the internal and external implant surfaces in individuals with periimplant disease: A cross-sectional study Abstract O bj e cti ve The objective of this study was to investigate the prevalence of Staphylococcus aureus (S. aureus) at internal and external dental implantsurfacesinpatientswithperiimplantdisease. Mate r i als and me tho ds Samplesformicrobiological analysiswere obtainedfromfourtypes ofsitesinthefollowingorder:(1)theperiimplantsulcularfluid(PISF) ofeachimplant;(2)thegingivalsulcus(GS)oftheadjacentteeth;(3) theimplant–abutmentconnectionandabutmentinnerportions(IIP) of each implant; and (4) the oropharyngeal complex (OF)—oral, tongueandpharynxswabswerealsocollected. Quantitative real-time polymerase chain reaction assays were carried out for total bacterial counts. The Kruskal–Wallis test was usedtocomparetheS. aureuslevelsatthevarioussites. Re su lts Mean bacterial counts ofS. aureuswere asfollows: GS = 5.02 × 102 ; PISF = 0,IIP = 0andOF = 0.Apositivevaluewasfoundforoneoutof the 35 sitesforeach group, but underthe limit ofquantification. For GS, one out of the 35 sites presented with a total bacterial count of 2.11 × 104 .Nostatisticallysignificantdifferenceswerefoundamong groupsregardingsitelocation(p= 0.40). Co nclu si o n Withinthelimitsofthisstudy,S. aureuscouldnotbequantifiedinthe PISFandinsidetheIIPaffectedbyperiimplantitis. Ke y wo rds Periimplantitis, periimplant disease, microbiological analysis, opportunistic pathogens, implant connection, S. aureus. Luigi Canullo,* Paulo Henrique Orlato Rossetti,† Marco Tallarico* & David Peñarrocha Oltra‡ * Independent researcher, Rome, Italy † Independent researcher, Bauru, Brazil ‡ Oral Surgery Unit, Department of Stomatology, Faculty of Medicine and Odontology, University of Valencia, Valencia, Spain Corresponding author: Dr. Luigi Canullo Via Nizza 46 00198 Rome Italy T + 39 347 620 1976 F + 39 06 841 1980 luigicanullo@yahoo.com How to cite this article: Canullo L, Orlato Rossetti PH, Tallarico M, Peñarrocha Oltra D. Identification of Staphylococcus aureus at the internal and external implant surfaces in individuals with periimplant disease: a cross-sectional study. J Oral Science Rehabilitation. 2016 Mar;2(1):8–13. T + 393476201976 F + 39068411980